viability assay kit  (Biotium)

Journal: Bioactive Materials

Article Title: A composite hydrogel enables the spatiotemporal delivery of distinct cytokines to drive the native vascularized bone regeneration

doi: 10.1016/j.bioactmat.2026.02.048

Figure Lengend Snippet: Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. A) Calcein/PI staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.

Article Snippet: A Calcein/PI Cell Viability/Cytotoxicity Assay Kit (Beyotime, China) was used to recognize the living and dead cells.

Techniques: In Vitro, Staining, Migration, Negative Control, Cell Culture, Software, Control

Journal: iScience

Article Title: Ready-to-use 3D bioprinted scaffolds from natural materials loaded with patient’s PRGF for personalized skin regeneration

doi: 10.1016/j.isci.2026.116185

Figure Lengend Snippet: Cell-scaffold interaction (A) Live/Dead staining of L-929 fibroblast cultures after 3 and 7 days in 2D conditions and on top of RTU scaffolds rehydrated with PBS and PRGF, respectively (scale bars, 100 μm). Biocompatibility assessment following ISO 10993 standards, evaluated by both direct contact (B) and extract-based (C) assays on L-929 fibroblasts. Cell adhesion and proliferation: SEM imaging and quantification of HDF fibroblast adhesion 24 h post-seeding on the scaffolds (D), followed by proliferation assessment after 72 h (E). Statistical significance is indicated as follows: not significant (ns); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

Article Snippet: LIVE/DEAD® viability/cytotoxicity kit , Fisher Scientific , Cat# L3224.

Techniques: Staining, Imaging

Journal: Materials Today Bio

Article Title: Rational design of FAP-targeted sEVs delivered by microneedles for precision treatment of hypertrophic scars via ferroptosis in hypertrophic scar fibroblasts

doi: 10.1016/j.mtbio.2026.103117

Figure Lengend Snippet: In vitro antifibrotic effects of sEVs ErF on HSFs. (A, B) EdU staining and quantification of proliferating HSFs treated with Con, Er, sEVs Er , or sEVs ErF . Scale bar, 100 μm. (C) CCK-8 assay showing dose-dependent viability changes. (D, E) Transwell migration images and quantification of migrated HSFs. Scale bar, 100 μm. (F, G) Wound healing assay images and migration area (%) over time. Scale bar, 400 μm. (H-K) Western blot and densitometric analysis of COL I, COL III, and α-SMA; GAPDH, loading control. (L, M) α-SMA immunofluorescence and quantitative fluorescence intensity in HSFs. Scale bar, 50 μm. (N, O) Calcein-AM/PI flow cytometry and quantification of PI-positive cells. Data are mean ± SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Cell viability and death were assessed using a Calcein-AM/PI Cell Viability/Cytotoxicity Kit (Beyotime, China).

Techniques: In Vitro, Staining, CCK-8 Assay, Migration, Wound Healing Assay, Western Blot, Control, Immunofluorescence, Fluorescence, Flow Cytometry